Recombinant PPM1G Monoclonal Antibody (AN300229P)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, 293T, Jurkat Verified Samples in IHC: Human heart, Human epididymis |
| Dilution | WB 1:500-1:2000, IHC-P 1:100-1:500, ICC/IF 1:20-1:100, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human PPM1G protein |
| Abbre | PPM1G |
| Synonyms | PPM1G, PP2CG, PP2CGAMMA, PPP2CG, PP2C-Gamma, Protein Phosphatase 1C, Protein Phosphatase 1G, Protein Phosphatase 2C Isoform Gamma, Protein Phosphatase Magnesium-Dependent 1 Gamma, PPM1C, MGC1675, MGC2870, OTTHUMP00000123426, PP2C, PP2C gamma, PPM1G_HUMAN, Protein phosphatase 2 catalytic subunit gamma isoform, Protein phosphatase 2C gamma isoform, Protein phosphatase magnesium dependent 1 gamma, Protein phosphatase Mg2+/Mn2+ dependent 1G |
| Swissprot | |
| Calculated MW | 59 kDa |
| Observed MW |
80 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Epigenetics and Nuclear Signaling |
| Clone No. | 3F3 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP1C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. |
Other Clones
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Unconjugated
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