Recombinant PMVK/phosphomevalonate kinase Monoclonal Antibody (AN300282P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2 Verified Samples in IP: Hela, NIH-3T3 |
| Dilution | WB 1:500-1:1000, IP 0.2-1 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human PMVK / phosphomevalonate kinase Protein |
| Abbre | PMVK |
| Synonyms | POROK, OTTHUMP, PMVK, HUMPMKI, PMK, PMKA, PMKASE, POROK1, hPMK, OTTHUMP00000035546, Phosphomevalonate kinase, PMK PEN, PMKPEN, PMKI |
| Swissprot | |
| Calculated MW | 21 kDa |
| Observed MW |
21 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | 14B11 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 2-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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