Recombinant PKM Monoclonal Antibody (AN301098L)
For research use only.
| Verified Samples |
Verified Samples in WB: A549 Verified Samples in IHC: Human kidney tissue, Mouse lung tissue |
| Dilution | IHC 1:200-1000, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PKM protein |
| Abbre | PKM |
| Synonyms | OIP, THBP, HEL-S, CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, PKM, p58, PK2, CTHBP, OIP3, p58, PKM2, TCB, THBP1, Cytosolic thyroid hormone binding protein, Cytosolic thyroid hormone-binding protein, Cytosolic thyroid hormone-binding protein (CTHBP), KPYM, MGC3932, muscle 2, muscle type, OIP 3, OIP-3, OPA interacting protein 3, Opa-interacting protein 3, Opa-interacting protein 3 (OIP-3), PK muscle type, pykm, Pyruvate kinase, Pyruvate kinase 2/3, Pyruvate kinase 3, Pyruvate kinase isozymes M1/M2, pyruvate kinase M1/2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, pyruvate kinase PKM, Threonine-protein kinase PKM2, Thyroid hormone binding protein 1, Thyroid hormone binding protein cytosolic, Thyroid hormone-binding protein 1, Thyroid hormone-binding protein 1 (THBP1), Tumor M2 PK, Tumor M2-PK, PKM2 |
| Swissprot | |
| Calculated MW | 57 kDa |
| Observed MW |
57 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism, Cardiovascular |
| Clone No. | 6A7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. |
Other Clones
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Unconjugated
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