Recombinant PI3 Kinase Class 3 Monoclonal Antibody (AN300641L)
For research use only.
| Verified Samples | Verified Samples in WB: Rat brain |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PI3 Kinase Class 3 protein |
| Abbre | PI3 Kinase Class 3 |
| Synonyms | VPS, hVps, PIK3C, PIK3C3, VPS34, hVps34, MGC61518, Phosphatidylinositol 3 kinase catalytic subunit type 3, Phosphatidylinositol 3 kinase class 3, Phosphatidylinositol 3 kinase p100 subunit, Phosphatidylinositol 3-kinase catalytic subunit type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide 3 kinase class 3, Phosphoinositide-3-kinase class 3, PI3 kinase type 3, PI3K type 3, PI3-kinase type 3, PK3C3, PtdIns 3 kinase type 3, PtdIns-3-kinase type 3, Vps 34, PI 3 Kinase Class 3 |
| Swissprot | |
| Calculated MW | 102 kDa |
| Observed MW |
102 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Midbody, Late endosome, Cytoplasmic vesicle, autophagosome, As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Immunology, Metabolism |
| Clone No. | 8D1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.,cofactor:Manganese.,Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 PI3K/PI4K domain.,subunit:Probably forms a complex with AMBRA1 and BECN1 (By similarity). Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4.,tissue specificity:Ubiquitously expressed, with a highest expression in skeletal muscle. |
Other Clones
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Unconjugated
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