Recombinant PHPT1 Monoclonal Antibody (AN300256P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, THP1, MCF7 Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:2000, IP 1-5 µL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human PHPT1 protein |
| Abbre | PHPT1 |
| Synonyms | PHPT, HSPC, Phosphohistidine phosphatase, CGI, PHPT1, CGI-202, HEL-S-132P, HSPC141, PHP, PHP14, 14 kDa phosphohistidine phosphatase, CGI 202, Phosphohistidine phosphatase 1, Protein janus A homolog, Protein janus-A homolog, Sex regulated protein janus a |
| Swissprot | |
| Calculated MW | 14 kDa |
| Observed MW |
14 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Clone No. | 11A5 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.2 channels. Alternative splicing results in multiple transcript variants. |
Other Clones
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Other Formats
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Unconjugated
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