Recombinant Phospho-VEGF Receptor 2 (Tyr1175) Monoclonal Antibody (AN300385L)
For research use only.
| Verified Samples | Verified Samples in WB:?HUVEC |
| Dilution | WB 1:1000-1:10000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the residues around Tyr1175 of Human Phospho-VEGF Receptor 2 |
| Abbre | KDR |
| Synonyms | Krd, VEGF Receptor, Fetal liver kinase, Protein-tyrosine kinase receptor flk, Vascular endothelial growth factor receptor, sVEGFR, VEGF R, 6130401C, FLK, CD309, FLK1, VEGFR, VEGFR2, VEGF Receptor 2, KDR, 6130401C07, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, Krd-1, Ly73, Protein-tyrosine kinase receptor flk-1, sVEGFR-2, Vascular endothelial growth factor receptor 2, VEGF R2, VEGFR-2, KDR |
| Swissprot | |
| Calculated MW | 152 kDa |
| Observed MW |
280 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane、Secreted |
| Tissue Specificity | Detected in cornea (at protein level). Widely expressed. |
| Concentration | 1 mg/mL |
| Buffer | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5 |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Microbiology, Signal Transduction, Stem Cells, Cancer, Metabolism |
| Clone No. | 11G9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. |
Other Clones
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