Recombinant Phospho-Smad2 (Ser250) Monoclonal Antibody (AN300960L)
For research use only.
| Verified Samples | Verified Samples in WB: A549 treated with TPA for 48 hours |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Ser250) of Human Phospho-Smad2 |
| Abbre | Smad2 |
| Synonyms | MADH, MADR, hMAD, SMAD, hSMAD, JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2, Smad2, Drosophila, homolog of, hSMAD family member 2, JV181, MAD, MAD homolog 2, MAD Related Protein 2, Mad-related protein 2, MGC22139, MGC34440, Mother against DPP homolog 2, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, OTTHUMP00000163489, Sma and Mad related protein 2, Sma- and Mad-related protein 2 MAD, SMAD 2, SMAD family member 2 |
| Swissprot | |
| Calculated MW | 58 kDa |
| Observed MW |
58 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nuclear |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Stem Cells, Cancer, Kits, Lysates, Other, Metabolism |
| Clone No. | 5F10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. |
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Unconjugated
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