Recombinant Phospho-p70 S6 kinase β (Ser371) Monoclonal Antibody (AN301151L)
For research use only.
| Verified Samples | Verified Samples in WB: HT-29 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Ser371) of Human Phospho-p70 S6 kinase β |
| Abbre | p70 S6 kinase β |
| Synonyms | S6K, RPS6KB, S6K-beta, RPS6KB2, KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, SRK, STK14B, p70(S6K)-beta, 70 kDa ribosomal protein S6 kinase 2, EC 2.7.11.1, KS6B2, p70 beta, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6KB, p70 S6Kbeta, p70 S6K-beta, p70(S6K) beta, p70-S6K 2, P70S6K2, p70S6Kb, polypeptide 2, Ribosomal protein S6 kinase 70kDa, Ribosomal protein S6 kinase B2, Ribosomal protein S6 kinase beta 2, Ribosomal protein S6 kinase beta-2, S6 kinase related kinase, S6 kinase-related kinase, S6K beta, S6K beta 2, S6K-beta-2, Serine/threonine protein kinase 14 beta, Serine/threonine-protein kinase 14B, P70S6k |
| Swissprot | |
| Calculated MW | 53 kDa |
| Observed MW |
60 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Metabolism |
| Clone No. | 6H12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | RPS6KB2(ribosomal protein S6 kinase B2) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. |
Other Clones
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Unconjugated
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