Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (AN300364L)
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For research use only.
| Verified Samples |
Verified Samples in WB:?NIH-3T3 Verified Samples in IHC: Human carcinoma of sigmoid Verified Samples in IF: NIH-3T3 |
| Dilution | WB 1:1000-1:10000, IHC-P 1:200-1:1000, ICC/IF 1:20-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | A synthetic phosphopeptide corresponding to residues around Thr180, Tyr182 of human Phospho-p38 MAPK. |
| Abbre | MAPK14 |
| Synonyms | Mxi, MAPK, CSPB, MAP kinase MXI, MAX-interacting protein, Mitogen-activated protein kinase, MAPK14, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, p38 MAPK, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase 14, CSAID binding protein, CSAID Binding Protein 1, CSAID-binding protein, Csaids binding protein, CSBP 1, CSBP 2, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase 14, MAP kinase p38 alpha, MAPK 14, MAX interacting protein 2, Mitogen Activated Protein Kinase 14, Mitogen activated protein kinase p38 alpha, Mitogen-activated protein kinase p38 alpha, MK14, Mxi 2, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, p38alpha Exip, p38-α |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. |
| Concentration | 1 mg/mL |
| Buffer | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5 |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Immunology |
| Clone No. | 12G13 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
Other Clones
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Unconjugated
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