Recombinant Phospho-FAK (Tyr397) Monoclonal Antibody (AN301150L)
For research use only.
| Verified Samples | Verified Samples in WB: Hela |
| Dilution | WB 1:2000-1:10000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Tyr397) of Human Phospho-FAK |
| Abbre | FAK |
| Synonyms | PTK, PPP1R, FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, PTK2, FADK 1, FAK related non kinase polypeptide, Focal adhesion Kinase, Focal adhesion kinase 1, Focal adhesion kinase isoform FAK Del33, Focal adhesion kinase related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein tyrosine kinase 2, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2 |
| Swissprot | |
| Calculated MW | 119 kDa |
| Observed MW |
119 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Nucleus. Cytoplasm, cytoskeleton, cilium basal body, Constituent of focal adhesions. Detected at microtubules. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Cancer |
| Clone No. | 6H11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | PTK2(protein tyrosine kinase 2) Homo sapiens This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. |
Other Clones
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Unconjugated
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