Recombinant Phospho-CREB (Ser133) Monoclonal Antibody (AN300123L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human breast cancer, Human lung cancer, Human renal carcinoma |
| Dilution | WB 1:10000-1:200000, IHC-P 1:100-1:500, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic phosphopeptide corresponding to residues around Ser133 of human CREB. |
| Abbre | CREB1 |
| Synonyms | CREB1, CREB, CREB-1, Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
38 kDa, 40 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5 |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Immunology, Signal Transduction |
| Clone No. | 9D15 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. |
Other Clones
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Unconjugated
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