Recombinant PGC1 β Monoclonal Antibody (AN300994L)
For research use only.
| Verified Samples | Verified Samples in WB: MCF7 |
| Dilution | WB 1:1000-1:5000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PGC1 β protein |
| Abbre | PGC1 β |
| Synonyms | PGC, PPARGC, 6-pyruvoyl tetrahydrobiopterin synthase, PTP synthase, PTPS, PTS, PERC, PGC1, PGC1B, PPARGC1, PPARGC1B, coactivator 1, coactivator 1 beta, gamma, peroxisome proliferative activated receptor, peroxisome proliferator-activated receptor gamma, peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC-1(beta), PGC-1-beta, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator-1beta, PPAR-gamma coactivator 1-beta, PPARGC-1-beta, PRGC2 |
| Swissprot | |
| Calculated MW | 113 kDa |
| Observed MW |
113 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cardiovascular, Metabolism |
| Clone No. | 11B11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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