Recombinant Peroxiredoxin 5/PRDX5 Monoclonal Antibody (AN300395P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?293T, Jurkat, 293, PRDX5 konckout Hela, Hela Verified Samples in IF: Hela Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:2000, ICC/IF 1:20-1:100, IP 4-8 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Peroxiredoxin 5/PRDX5 protein |
| Abbre | PRDX5 |
| Synonyms | PMP, ACR, AOEB, SBBI, Peroxiredoxin, Alu corepressor, Antioxidant enzyme B, Thioredoxin peroxidase PMP, HEL-S, PRDX5, ACR1, AOEB166, B166, HEL-S-55, PLP, PMP20, PRDX6, PRXV, SBBI10, prx-V, Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxiredoxin V, Peroxiredoxin-5, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI, Alu co repressor 1, epididymis secretory protein Li 55, mitochondrial, peroxiredoxin 5, PRDX 5 |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
15 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Widely expressed. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cancer, Metabolism |
| Clone No. | 12F12 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. |
Other Clones
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Unconjugated
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