Recombinant Periostin Monoclonal Antibody (AN300918L)
For research use only.
| Verified Samples | Verified Samples in WB: T47D |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Periostin protein |
| Abbre | Periostin |
| Synonyms | POSTN/OSF, RP11-412K, Osteoblast Specific Factor, Osteoblast-specific factor, OSF, POSTN, OSF-2, OSF2, PDLPOSTN, PN, periostin, Osteoblast Specific Factor 2, Osteoblast-specific factor 2, POSTN/OSF-2, RP11-412K4.1, Periodontal ligament specific periostin, OSF2, Osteoblast specific factor 2, PDLPOSTN, Periostin, POSTN, Fasciclin-I like, MGC119510, MGC119511, OSF 2, Osteoblast specific factor 2 (fasciclin I like), Periodontal ligament specific periostin, Periostin isoform thy2, Periostin isoform thy4, Periostin isoform thy6, Periostin isoform thy8, Periostin osteoblast specific factor, RP11 412K4.1, POSTN |
| Swissprot | |
| Calculated MW | 93 kDa |
| Observed MW |
93 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Tags & Cell Markers, Cancer, Cardiovascular |
| Clone No. | 9C12 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Other Clones
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Unconjugated
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