Recombinant PD L2 Monoclonal Antibody (E-AB-81595)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, C6 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Rat |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic peptide of human PD-L2 |
| Abbre | PD L2 |
| Synonyms | B7 dendritic cell molecule, B7-DC, B7DC, Btdc, Butyrophilin B7 DC, Butyrophilin B7-DC, Butyrophilin B7DC, CD 273, CD273, CD273 antigen, MGC142238, MGC142240, PD 1 ligand 2, PD L2, PD-1 ligand 2, PD-L2, PD1 ligand 2, PD1L2, PDCD 1 ligand 2, PDCD1 ligand 2, PDCD1L2, bA574F11.2 |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
31 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cell membrane and Endomembrane system. |
| Concentration | 300 μg/mL |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
| Purification Method | Affinity Purified |
| Research Areas | Immunology |
| Clone No. | R02-2A1 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by thePDCD1LG2 gene.PDCD1LG2 has also been designated as CD273 (cluster of differentiation 273).Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner.Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. |
Other Clones
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Other Formats
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Unconjugated
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