Recombinant Osteopontin Monoclonal Antibody (AN300896L)
For research use only.
| Verified Samples | Verified Samples in WB: A549 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Osteopontin protein |
| Abbre | Osteopontin |
| Synonyms | BSP, Bone sialoprotein, secreted phosphoprotein, Early T-Lymphocyte Activation, PSEC, SPP, ETA, BNSP, BSPI, ETA-1, OPN, Osteopontin, SPP1, Bone Sialoprotein I, BSP1, Early T-Lymphocyte Activation 1, SPP-1, Nephropontin, Uropontin, Bone sialoprotein 1, ETA 1, secreted phosphoprotein 1, SPP 1, Urinary stone protein, PSEC0156, OPN |
| Swissprot | |
| Calculated MW | 35 kDa |
| Observed MW |
55,65 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Tissue Specificity | Bone, Found in plasma |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Stem Cells, Cancer |
| Clone No. | 7C9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | SPP1(secreted phosphoprotein 1) Homo sapiens The protein encoded by this gene is involved inThe attachment of osteoclasts toThe mineralized bone matrix.The encoded protein is secreted and binds hydroxyapatite with high affinity.The osteoclast vitronectin receptor is found inThe cell membrane and may be involved inThe binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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