Recombinant ORP150/ORP150/HYOU1/HSP12A Monoclonal Antibody (AN300399P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?293T, Hela Verified Samples in IHC: Human colon carcinoma, Human cirrhosis Verified Samples in IF: MCF7 Verified Samples in IP: MCF-7 |
| Dilution | WB 1:500-1:2000, IHC-P 1:100-1:500, ICC/IF 1:20-1:100, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human ORP150/HYOU1/HSP12A protein |
| Abbre | HYOU1 |
| Synonyms | HYOU, Hypoxia up-regulated protein, Grp, ORP, HYOU1, GRP-170, Grp170, HSP12A, ORP-150, ORP150, 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, Hypoxia up-regulated protein 1 |
| Swissprot | |
| Calculated MW | 111 kDa |
| Observed MW |
170 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Neuroscience, Epigenetics and Nuclear Signaling, Cancer, Metabolism |
| Clone No. | 9D5 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. |
Other Clones
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