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Recombinant NT5C3A/UMPH1 Monoclonal Antibody (AN300444P)

Recombinant NT5C3A/UMPH1 Monoclonal Antibody - 1
  • Recombinant NT5C3A/UMPH1 Monoclonal Antibody - 1
  • Recombinant NT5C3A/UMPH1 Monoclonal Antibody - 2
  • Recombinant NT5C3A/UMPH1 Monoclonal Antibody - 3
All Size Price Qty
100μL $ 380.00
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For research use only.

Verified Samples Verified Samples in WB:?HepG2
Verified Samples in IP: 293T
Dilution WB 1:500-1:1000,  IP 1-2 μL/mg of lysate
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IP
Clonality Monoclonal
Immunogen Recombinant Human NT5C3A/UMPH1 Protein
Abbre NT5C3A
Synonyms PSN,  NT5C,  hUMP,  HSPC,  P5N,  NT5C3A,  NT5C3,  P5N-1,  PN-I,  POMP,  PSN1,  UMPH,  UMPH1,  cN-III,  hUMP1,  p36,  P5N1,  HSPC233
Swissprot
Calculated MW 38 kDa
Observed MW 39 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Tissue Specificity Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS
Purification Method Protein A
Research Areas Epigenetics and Nuclear Signaling
Clone No. 2D5
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4.
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Unconjugated

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