Recombinant NME1/NDKA Monoclonal Antibody (AN300066P)
For research use only.
| Verified Samples | Verified Samples in WB: Hela, Jurkat, HepG2 |
| Dilution | WB 1:500-1:2000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human NME1 / NDKA protein |
| Abbre | NME1 |
| Synonyms | NME, Metastasis Inhibition Factor nm, NM23-H, NME1, AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1, Granzyme A-Activated DNase, Metastasis Inhibition Factor nm23, NDK A, NDP Kinase A, Nucleoside Diphosphate Kinase A, Tumor Metastatic Process-Associated Protein, drosophila, Granzyme A activated DNase, GZMA activated DNase, homolog 1, homolog of, included, NM23 long variant, NM23H1B, NM23-M1, NME/NM23 nucleoside diphosphate kinase 1, NME1-NME2 spliced read-through transcript, Nonmetastatic cells 1, Non-metastatic cells 1, Nonmetastatic protein 23, protein (NM23A) expressed in, protein expressed in |
| Swissprot | |
| Calculated MW | 17 kDa |
| Observed MW |
20 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | 6A10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME1), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
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