Recombinant NDRG1 Monoclonal Antibody (AN300663L)
For research use only.
| Verified Samples | Verified Samples in WB: RAW264.7 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human NDRG1 protein |
| Abbre | NDRG1 |
| Synonyms | NDR, CAP, RIT, TDD, NDRG, TARG, protein NDRG, Nickel-Specific Induction Protein Cap, PROXY, DRG, NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, protein NDRG1, Differentiation-Related Gene 1 Protein, Nickel-Specific Induction Protein Cap43, N-myc Downstream-Regulated Gene 1 Protein, Reducing Agents and Tunicamycin-Responsive Protein, CAP43, CMT4D, DRG1, DRG-1, GC4, HMSNL, NDR1, NMSL, protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5, 42 kDa, Anti GC4, Differentiation related gene1 protein, Drg 1, Human mRNA for RTP complete cds, N myc downstream regulated gene 1, N myc downstream regulated gene 1 protein, Ndr 1, NDRG 1, NDRG1 protein, Nickel specific induction protein, Nickel specific induction protein Cap43, Nmyc downstream regulated, Nmyc downstream regulated gene1, Nmyc downstream regulated gene1 protein, Protein regulated by oxygen 1, Protein regulated by oxygen1, Reduced in tumor, Reducin, Reducing agents and tunicamycin responsive protein, tdds, Tunicamycin responsive protein |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW |
48 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 1F15 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
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