Recombinant NCAM1/CD56 Monoclonal Antibody (AN300292P)
For research use only.
| Verified Samples | Verified Samples in WB:?Jurkat, SH-SY5Y, Mouse brain |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the Human NCAM1/CD56 |
| Abbre | NCAM1 |
| Synonyms | MSK, NKH, Leu, N-CAM, NCAM1 /CD, CD56, MSK39, NCAM, NCAM1, NCAM1 /CD56, NKH1, CD56 antigen, Leu-19, NCAM-1, N-CAM-1, neural cell adhesion molecule, Neural cell adhesion molecule 1, Leu-19, NKH-1, antigen MSK39 identified by monoclonal 5.1H11, antigen recognized by monoclonal 5.1H11, cell adhesion molecule, MSK 39, NCAM 1, NCAM C, NCAMC, neural, neural cell adhesion molecule, Neural cell adhesion molecule NCAM, OTTHUMP00000235666 |
| Swissprot | |
| Calculated MW | 93 kDa |
| Observed MW |
93 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane,Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Stem Cells |
| Clone No. | 2B13 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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