For research use only.
Verified Samples | Verified Samples in WB: Jurkat, C6, Hela Verified Samples in IF: Hela |
Dilution | WB 1:500-1:1000, IF 1:100-1:200 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human, Rat |
Applications | WB, IF |
Clonality | Rabbit Monoclonal |
Immunogen | Recombinant protein of human NAT10 |
Abbre | NAT10 |
Synonyms | ALP, DKFZp434C116, FLJ10774, FLJ12179, FLJ23850, KIAA1709, N acetyltransferase 10, N acetyltransferase 10 GCN5 related, N acetyltransferase like, N acetyltransferase like protein, N-acetyltransferase 10, NAT10, NET43, hALP |
Swissprot | |
Calculated MW | 116 kDa |
Observed MW | 116 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus>nucleolus. Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase. |
Concentration | 300 μg/mL |
Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein. |
Purification Method | Affinity Purified |
Research Areas | Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction |
Clone No. | R05-1C8 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | NAT-10 (N-acetyltransferase 10) is a nuclear protein that belongs to the UPF0202 family. It has a single N-acetyltransferase domain that likely functions as a histone acetyltransferase. NAT10 functions primarily to regulate the activity of telomerase. It is upregulated in response to DNA damage and is likely to take part in genotoxic resistance and DNA repair. NAT-10 has a high binding potential for the promoter region of TERT which stimulates the production of telomerase. These varieties of function imply that human telomerase complexes have multiple functions rather than specific duties. |
Other Clones
1 Results
Other Formats
1 Results
Unconjugated
