Recombinant Mouse SH3Y1 Protein(Sumo Tag) (PDEM100137)
For research use only.
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Met1-Asn340 |
| Accession | O08641 |
| Calculated Molecular Weight | 50.3 kDa |
| Observed Molecular Weight | 55 kDa |
| Tag | N-Sumo |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Differential screening of mouse skin cDNA libraries identified cDNA encoding a protein containing a Src homologous 3 (SH3) domain, a novel protein containing a SH3 domain at the carboxyl terminus, and reporting gene expression patterns during the hair growth cycle. The protein shows significant homology with an the yeast protein YHRO 16c/Ysc 84 in its amino and carboxyl terminus, suggesting that it may be a mouse homolog of the yeast protein. Therefore, we name it the Sh3 domain YSC-like 1, Sh3yl1. |
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