Recombinant Mouse Interleukin-18/IL-18 Protein (His Tag) (PKSM041079)
For research use only.
| Synonyms | Interleukin, IL-1F, Ig, Il-Igif, Il-18, IFN-gamma-inducing factor, Il, IL-1 gamma, IL-1F4, IL-1g, Interferon gamma-inducing factor, Interferon-gamma-inducing factor, Interleukin-1 gamma, Interleukin-18, Igif, Il18, Igif, Il-, Il-18 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Asn36-Ser192 |
| Accession | P70380 |
| Calculated Molecular Weight | 19.1 kDa |
| Observed Molecular Weight | 18 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce IFN gamma secretion in KG-1 cells.The ED50 for this effect is <0.5 μg/mL. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Interleukin-18 (IL-18）is a protein which belongs to the IL-1 family. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively. IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex. Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils. |
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