Recombinant Mouse Galectin-3/Lgals3 protein (His Tag) (PDEM100233)
For research use only.
| Synonyms | 35 kDa lectin, CBP35, Carbohydrate binding protein 35, GAL3, GALBP, GALIG, GBP, Gal-3, IgE binding protein, LGALS2, Lectin L-29, Mac-2 antigen, Macrophage galactose-specific lectin |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Ala2-Ile264 |
| Accession | P16110 |
| Calculated Molecular Weight | 27.4 kDa |
| Observed Molecular Weight | 31-33 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes. |
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