Recombinant Mouse CXCL1 Protein(GST Tag) (PDEM100322)
For research use only.
| Synonyms | CXCL, SCYB, Secretory protein N, Neutrophil-Activating Protein, GROα/CXCL, C-X-C Motif Chemokine 1, GRO-A, GRO-Alpha(1-73), Growth-Regulated Alpha Protein, GROα/CXCL1, Melanoma Growth Stimulatory Activity, NAP3, Neutrophil-Activating Protein 3, Platelet-derived growth factor-inducible protein KC, Secretory protein N51, CINC1, CINC-1, CXCL1, FSP, GRO1, GROa, KC, MGSA, MGSA-a, MGSA-alpha, NAP-3, SCYB1, GRO alpha, Growth Regulated Oncogene Alpha, Gro |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Ala25-Lys96 |
| Accession | P12850 |
| Calculated Molecular Weight | 33.9 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
| Background | Growth-regulated alpha protein (CXCL1,KC), is a member of the alpha chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. The N-terminal processed form KC(5-72) of the protein is produced by proteolytic cleavage after secretion from bone marrow stromal cells, and shows a highly enhanced hematopoietic activity. Mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. Cxcl1 has chemotactic activity for neutrophils, and contributes to neutrophil activation during inflammation. Hematoregulatory chemokine, in vitro, suppresses hematopoietic progenitor cell proliferation. |
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