Recombinant Mouse Carboxylesterase 2E/CES2E Protein (His Tag) (PKSM040582)
For research use only.
| Synonyms | 1700081L16Rik, 1700122C07Rik, BB081581, Ces5, Ces7, Gm503, Pyrethroid hydrolase Ces2e, carboxylesterase 2E, cauxin |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-His 556 |
| Accession | NP_766347.1 |
| Calculated Molecular Weight | 60.6 kDa |
| Observed Molecular Weight | 52 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is > 50, 000 pmoles/min/μg. |
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Carboxylesterase belongs to the class of serine hydrolases family which includes Chymotrypsin and Acetylcholinesterase. Carboxylesterase is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Carboxylesterase is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it's widely distributed in cells especially in mammalian liver. Carboxylesterase 5 (CES5), also known as cauxin or CES7, is one of CES enzyme families exerting role in catalyzing hydrolytic and transesterfication reactions with broad substrat specifity. CES5 is reported in high concentrations in the urine of adult male cats, and within a protein complex of mammalian male epididymal fluids. Roles for carboxylesterase 5 may include regulating urinary levels of cat pheromone, catalyzing lipid transfer reactions within mammalian male reproductive fluids, and protecting neural tissue from drugs and xenobiotics. |
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