Recombinant Mouse Carbonic Anhydrase VIII/CA8 Protein (His Tag) (PKSM040495)
For research use only.
| Synonyms | AW546993, Ca8, Cals, Cals1, Car8, Carp, RP23-180H12.1, wdl |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Met 1-Gln 291 |
| Accession | P28651 |
| Calculated Molecular Weight | 34.5 kDa |
| Observed Molecular Weight | 37 kDa |
| Tag | C-His |
| Bio-activity | Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg. |
| Purity | > 88 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 50mM Tris, pH 8.0 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer. |
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