Recombinant MOB1 Monoclonal Antibody (AN301343L)

For research use only.
Verified Samples | Verified Samples in WB: MOLT-4 |
Dilution | WB 1:2000-1:10000, |
Isotype | IgG,κ |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | WB |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant Human MOB1 protein |
Abbre | MOB1 |
Synonyms | C2orf, MOB Kinase Activator 1A, Mob1 Alpha, Mob1 Homolog 1B, Mps One Binder Kinase Activator-like 1B, C2orf6, MOB4B, MOBK1B, MOBKL1B, MOB1A, Chromosome 2 open reading frame 6, FLJ10788, FLJ11595, MATS1, MOB1, MOB1 Mps One Binder homolog A, MOB1 Mps One Binder kinase activator like 1B, Mob4B protein, MOL1B, Mps one binder kinase activator like 1B, Mps One Binder kinase activator like 1B (yeast), Protein Mob4B |
Swissprot | |
Calculated MW | 24 kDa |
Observed MW |
24 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm, Nucleus |
Concentration | 0.2 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A |
Research Areas | Signal Transduction, Cancer |
Clone No. | 7A2 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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