Recombinant MDR1 Monoclonal Antibody (AN300819L)
For research use only.
| Verified Samples | Verified Samples in WB: SH-SY5Y |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human MDR1 protein |
| Abbre | MDR1 |
| Synonyms | MDR, PGY, ABC, ABCB, P-glycoprotein, Phospholipid transporter ABCB, ATP-dependent translocase ABCB, ATP-binding cassette sub-family B member, ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, P Glycoprotein, ABCB1, ATP-binding cassette sub-family B member 1, ATP-dependent translocase ABCB1, P-glycoprotein 1, Phospholipid transporter ABCB1, MDR-1, P-gp, gp170, ABC-B1, ATP binding cassette, Colchicin sensitivity, Doxorubicin resistance, member 1, Multidrug resistance 1, Multidrug resistance protein 1, P glycoprotein 1, P gp, sub family B (MDR/TAP) |
| Swissprot | |
| Calculated MW | 141 kDa |
| Observed MW |
141 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Stem Cells, Cancer |
| Clone No. | 5B3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. |
Other Clones
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Other Formats
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Unconjugated
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