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Recombinant LFA-3/CD58 Monoclonal Antibody (AN300251P)

All Size Price Qty
100μL $ 380.00
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For research use only.

Verified Samples Verified Samples in WB: Jurkat, K562
Verified Samples in IP: Jurkat
Dilution WB 1:500-1:1000,  IP 0.2-1 μL/mg of lysate
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IP
Clonality Monoclonal
Immunogen Recombinant Human LFA-3 / CD58 Protein
Abbre CD58
Synonyms LFA,  Ag3,  CD58 antigen,  Lymphocyte function-associated antigen 3,  Surface glycoprotein LFA-3,  LFA3,  CD58,  LFA-3
Swissprot
Calculated MW 28 kDa
Observed MW 61 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer 0.2 μm filtered solution in PBS
Purification Method Protein A
Research Areas Immunology
Clone No. 10A9
Conjugation Unconjugated
Storage This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Shipping Ice bag
background Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
Other Clones

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Unconjugated

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