Recombinant LCN2/NGAL Monoclonal Antibody (AN300307P)
For research use only.
| Verified Samples | Verified Samples in WB:?Jurkat |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human LCN2/NGAL protein |
| Abbre | LCN2 |
| Synonyms | LCN, 24p, Oncogene 24p, Siderocalin LCN, 25 kDa alpha-2-microglobulin-related subunit of MMP, LCN2, 24p3, MSFI, NGAL, p25, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin 2, Lipocalin-2, Neutrophil gelatinase-associated lipocalin, Oncogene 24p3, Siderocalin LCN2, HNL, Lipocalin 2, MSFI, Oncogene 24p3, 25 kDa alpha 2 microglobulin related subunit of MMP9, Alpha 2 microglobulin related protein, HGNC:6526, Lcn 2, Migration stimulating factor inhibitor, Neutrophil gelatinase associated lipocalin, Neutrophil gelatinase associated lipocalin precursor, Oncogenic lipocalin 24p3, Siderocalin, SV40 induced 24P3 protein, Uterocalin |
| Swissprot | |
| Calculated MW | 23 kDa |
| Observed MW |
23 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Detected in neutrophils (at protein level) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cardiovascular, Signal Transduction, Cancer, Metabolism |
| Clone No. | 7F9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. |
Other Clones
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Unconjugated
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