Recombinant KRT14/Cytokeratin 14 Monoclonal Antibody (AN300289P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?A431 Verified Samples in IHC: Human skin, Human esophageal carcinoma Verified Samples in IP: A431 |
| Dilution | WB 1:500-1:2000, IHC-P 1:10000-1:20000, IP 1-4 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, IP |
| Clonality | Monoclonal |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14. |
| Abbre | KRT14 |
| Synonyms | KRT, Cytokeratin, EBS, CK14, EBS3, EBS4, K14, NFJ, Cytokeratin 14, KRT14, ck14, Cytokeratin 14, EBS3, EBS4, K14, Krt14, NFJ, CK 14, CK-14, Cytokeratin14, Cytokeratin-14, Dowling Meara, Dowling-Meara, Epidermolysis bullosa simplex, K1C14, Keratin, Keratin 14, Keratin 14 (epidermolysis bullosa simplex, Keratin type I cytoskeletal 14, Keratin14, Keratin-14, Koebner, Koebner), Krt 14, OTTHUMP00000164624, type I cytoskeletal 14 |
| Swissprot | |
| Calculated MW | 52 kDa |
| Observed MW |
52 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Specificity | Expressed in the corneal epithelium (at protein level) |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 1A7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. |
Other Clones
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Unconjugated
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