Recombinant KLK-11/Kallikrein-11 Monoclonal Antibody (AN300204P)
For research use only.
| Verified Samples |
Verified Samples in WB: A549 Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:1000, IP 0.2-1 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human KLK-11 protein |
| Abbre | KLK11 |
| Synonyms | KLK, UNQ, PRO, PRSS, KLK11, PRSS20, TLSP, UNQ649, PRO1279, Hippostasin, hK11, Kallikrein 11, Kallikrein 11 isoform 1 preproprotein, Kallikrein 11 isoform 2 precursor, Kallikrein 11 precursor, Kallikrein related peptidase 11, Kallikrein-11, Kallikrein-11 inactive chain 2, KLK 11, MGC33060, Protease serine 20 trypsin like, Protease serine trypsin like, Serine protease 20, Trypsin like protease, Trypsin-like protease |
| Swissprot | |
| Calculated MW | 31 kDa |
| Observed MW |
49 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer, Cell Biology |
| Clone No. | 8C7 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 16. Alternate splicing and the use of alternate promoters results in multiple transcript variants encoding distinct isoforms which are differentially expressed. |
Other Clones
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Unconjugated
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