Recombinant JNK2/MAPK9 Monoclonal Antibody (AN300348P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?Jurkat Verified Samples in IHC: Human kidney, Human brain Verified Samples in IF: Hela Verified Samples in IP: HepG2 |
| Dilution | WB 1:500-1:1000, IHC-P 1:500-1:2500, ICC/IF 1:100-1:500, IP 1-2 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human JNK2/MAPK9 protein |
| Abbre | MAPK9 |
| Synonyms | MAPK, PRKM, JNK, MAPK9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK, c Jun kinase 2, C Jun N terminal kinase 2, c-Jun N-terminal kinase 2, JNK 55, JNK2 alpha, JNK2 beta, Jun kinase, MAP kinase 9, MAPK 9, Mitogen activated protein kinase 9, mitogen-activated, Mitogen-activated protein kinase 9, MK09, Protein kinase, SAPK alpha, Stress activated protein kinase 1a, Stress-activated protein kinase JNK2 |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
54 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cell Biology, Cancer, Immunology, Metabolism, Epigenetics and Nuclear Signaling |
| Clone No. | 6A9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. |
Other Clones
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Unconjugated
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