Recombinant JAK1 Monoclonal Antibody (AN300646L)
For research use only.
| Verified Samples | Verified Samples in WB: T47D |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human JAK1 protein |
| Abbre | JAK1 |
| Synonyms | JTK, Janus kinase, Tyrosine-protein kinase JAK, JAK, JAK1, JAK1A, JAK1B, JTK3, JAK 1, JAK 1A, JAK 1B, JAK-1, Janus kinase 1, Janus kinase 1 (a protein tyrosine kinase), Tyrosine protein kinase JAK 1, Tyrosine protein kinase JAK1, Tyrosine-protein kinase JAK1, JAK1, JAK1A |
| Swissprot | |
| Calculated MW | 133 kDa |
| Observed MW |
133 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endomembrane system; Peripheral membrane protein. Wholly intracellular, possibly membrane associated. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 8D6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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