Recombinant ISG15 Monoclonal Antibody (AN300127P)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela, 293, Daudi Verified Samples in IF: Hela |
| Dilution | WB 1:2000-1:10000, ICC/IF 1:20-1:100, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, ICC/IF |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human ISG15 protein |
| Abbre | ISG15 |
| Synonyms | ISG, G1P, IFI, IMD, Ubiquitin-Like Protein ISG, ISG15, G1P2, IFI15, IMD38, IP17, UCRP, hUCRP, Interferon-Induced 15 kDa Protein, Interferon-induced 17 kDa protein, ISG15 ubiquitin-like modifier, Ubiquitin Cross-Reactive Protein, Ubiquitin-Like Protein ISG15, alpha-inducible protein (clone IFI-15K), IFI 15, Interferon, Interferon alpha inducible protein, Interferon induced protein 15, Interferon induced protein IFI-15K, Interferon stimulated protein 15 kDa, Interferon-induced 17-kDa/15-kDa protein, ISG 15, ISG15 ubiquitin like modifier |
| Swissprot | |
| Calculated MW | 18 kDa |
| Observed MW |
15 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | 5D9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | ISG15 (interferon-induced 15 kDa protein; also known as IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15. |
Other Clones
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Unconjugated
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