Recombinant IRF-1 Monoclonal Antibody (AN301305L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse brain |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human IRF-1 protein |
| Abbre | IRF-1 |
| Synonyms | Interferon regulatory factor, Interferon regulatory factor 1 isoform d, Interferon regulatory factor 1 isoform +I, IRF, IRF1, IRF-1, MAR, MAR1, Interferon regulatory factor 1, Interferon regulatory factor 1 isoform +I9, Interferon regulatory factor 1 isoform d78, Interferon regulatory factor 1 isoform delta4, Interferon regulatory factor 1 isoform delta7, IRF 1, IRF 1 |
| Swissprot | |
| Calculated MW | 37 kDa |
| Observed MW |
48 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm, MYD88-associated IRF1 migrates into the nucleus more efficiently than non-MYD88-associated IRF1. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling, Cardiovascular |
| Clone No. | 7C5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor (IRF) family. IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes. IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma. Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion. |
Other Clones
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Unconjugated
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