Recombinant Integrin β3 Monoclonal Antibody (AN300876L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse spleen |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Integrin β3 protein |
| Abbre | Integrin β3 |
| Synonyms | ITGB, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, GT, Integrin beta 3, ITGB3, Integrin beta-3, &, beta-3 integrin-n, beta subunit, CD 61, CD61 antigen, HPA 1, HPA 4, Integrin beta 3 (platelet glycoprotein IIIa antigen CD61), Integrin beta chain beta 3, ITB3, ITG B3, ITGB 3, ITG-B3, NAIT, Platelet fibrinogen receptor, Platelet fibrinogen receptor beta subunit, Platelet glycoprotein IIIa, Platelet glycoprotein IIIa precursor, Platelet Membrane Glycoprotein IIIa, PTP |
| Swissprot | |
| Calculated MW | 87 kDa |
| Observed MW |
100 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Signal Transduction, Immunology, Stem Cells |
| Clone No. | 4D5 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | ITGB3(integrin subunit beta 3) Homo sapiens The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. |
Other Clones
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Unconjugated
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