Recombinant Integrin β1 Monoclonal Antibody (AN300858L)
For research use only.
| Verified Samples |
Verified Samples in WB: 3T3-L1 Verified Samples in IHC: Mouse stomach, Human hepatocellular carcinoma |
| Dilution | IHC 1:2000-1:10000, WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Integrin β1 protein |
| Abbre | Integrin β1 |
| Synonyms | MSK, MDF, ITB, ITGB, Integrin β, CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, Integrin beta 1, ITGB1, Integrin β1, Fibronectin receptor subunit beta, Integrin beta-1, ITB1, VLA-4 subunit beta, integrin &, beta-1-1, gpIIa, antigen CD29 includes MDF2, beta 1 (fibronectin receptor, beta polypeptide, beta1 integrin, Glycoprotein IIa, GP IIa, Integrin, Integrin subunit beta 1, integrin VLA-4 beta subunit, MSK12), OTTHUMP00000019420, Very late activation protein, VLA BETA, VLAbeta |
| Swissprot | |
| Calculated MW | 88 kDa |
| Observed MW |
115-125 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer, Metabolism, Neuroscience, Microbiology, Stem Cells, Developmental Biology |
| Clone No. | 7B4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. |
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Unconjugated
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