Recombinant iNOS Monoclonal Antibody (AN300597L)
For research use only.
| Verified Samples | Verified Samples in WB: RAW264.7 |
| Dilution | WB 1:2000-1:10000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human iNOS protein |
| Abbre | iNOS |
| Synonyms | NOS, INOS, NOS2A, HEP-NOS, NOS2, I-NOS, inducible, Nitric oxide synthase, Hepatocyte NOS, NOS type II, Hepatocyte, hepatocytes), HEPNOS, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, MAC NOS, macrophage, Macrophage NOS, Nitric oxide synthase 2 inducible, Nitric oxide synthase 2 inducible macrophage, nitric oxide synthase 2A (inducible, Nitric oxide synthase inducible, NOS 2, Nos II, Peptidyl-cysteine S-nitrosylase NOS2 |
| Swissprot | |
| Calculated MW | 131 kDa |
| Observed MW |
131 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, cytosol, Localizes as discrete foci scattered throughout the cytosol and in the presence of SPSB1 and SPSB4, exhibits a more diffuse cytosolic localization. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Cancer, Metabolism |
| Clone No. | 1A7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
Other Clones
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Unconjugated
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