Recombinant IL18 Monoclonal Antibody (AN301078L)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IHC: Human tonsil tissue, Mouse spleen tissue |
| Dilution | IHC 1:200-1000, WB 1:1000-5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human IL18 protein |
| Abbre | IL18 |
| Synonyms | MGC, IL1F, IL-1F, IL18, IGIF, IL-18, IL-1g, IL1F4, GIF, Iboctadekin, IFN-gamma-inducing factor, IL-1 gamma, IL-1F4, Interferon gamma-inducing factor, Interferon-gamma-inducing factor, Interleukin 18, Interleukin-1 gamma, Interleukin-18, MGC12320, IFN gamma inducing factor, IL 1 gamma, IL 18, IL 1g, IL1 gamma, IL18 protein, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interleukin 1 gamma, Interleukin 18 (interferon-gamma-inducing factor), Interleukin18 |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
22 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer, Metabolism |
| Clone No. | 2C4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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