Recombinant IL-33 Monoclonal Antibody (AN300184P)
For research use only.
| Verified Samples | Verified Samples in IHC: Human stomach, Human tonsil |
| Dilution | IHC-P 1:500-1:2000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | IHC-P |
| Clonality | Rabbit Monoclonal |
| Immunogen | Recombinant Human IL-33 Protein |
| Abbre | IL33 |
| Synonyms | DVS, IL1F, Interleukin, C9orf, IL-1F, IL33, C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, DVS27 related protein, IL-1F11, IL-33, Interleukin-1 family member 11, Interleukin-33, Nuclear factor from high endothelial venules, NFHEV, CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, IL 1F11, IL 33, Interleukin 1 family member 11, Interleukin 33, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, Interleukin-33 (109-270), NF HEV, Nuclear factor for high endothelial venules, OTTHUMP00000021041, RP11 575C20.2 |
| Swissprot | |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Immunology |
| Clone No. | 4D10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription. DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries. NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues. IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation. Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment. The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses. A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP. Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Full length and mature human IL-33 share 52 ‑ 58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins. |
Other Clones
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Unconjugated
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