Recombinant Human UBE2T Protein (His Tag) (PKSH033331)
For research use only.
| Synonyms | Cell Proliferation-Inducing Gene 50 Protein, UBE2T, Ubiquitin Carrier Protein T, Ubiquitin-Conjugating Enzyme E2 T, Ubiquitin-Protein Ligase T |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Val197 |
| Accession | Q9NPD8 |
| Calculated Molecular Weight | 24.7 kDa |
| Observed Molecular Weight | 25-30 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | Ubiquitin-Conjugating Enzyme E2 T (UBE2T) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2T accepts the ATP-dependent ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2T is able to catalyze polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11'-; 'Lys-27'-; 'Lys-48'- and 'Lys-63'-linked polyubiquitination. UBE2T is an important factor of the Faconi anemia pathway of DNA damage repair and; upon self-inactivation; may negatively regulate the Faconi pathway. |
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