Recombinant Human UBE2M/UBC12 Protein (PKSH033478)
For research use only.
| Synonyms | NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC12, UBE2M, Ubiquitin-conjugating enzyme E2 M |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met1-Lys183 |
| Accession | P61081 |
| Calculated Molecular Weight | 20.9 kDa |
| Observed Molecular Weight | 25 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 2mM DTT, 150mM NaCl, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This protein is linked with a ubiquitin-like protein; NEDD8; which can be conjugated to cellular proteins; such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; but not RBX2; suggests that the RBX1-UBE2M complex neddylates specific target proteins; such as CUL1; CUL2; CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. |
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