Recombinant Human UBE2D4 Protein (GST Tag) (PKSH033309)

For research use only.
Synonyms | HBUCE1, UBCH5D, UBE2D4, Ubiquitin Carrier Protein D4, Ubiquitin-Conjugating Enzyme E2 D4, Ubiquitin-Protein Ligase D4 |
Species | Human |
Expression Host | E.coli |
Sequence | Met 1-Met 147 |
Accession | Q9Y2X8 |
Calculated Molecular Weight | 43.5 kDa |
Observed Molecular Weight | 40 kDa |
Tag | N-GST |
Bio-activity | Not validated for activity |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Reconstitution | Not Applicable |
Background | Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification; protein K6-linked ubiquitination; protein K11-linked ubiquitination; protein K27-linked ubiquitination; protein K29-linked ubiquitination; protein K48-linked ubiquitination; and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro; UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues; but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination. |
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