Recombinant Human UBE2D1 Protein (GST Tag) (PKSH033367)
For research use only.
| Synonyms | SFT, Stimulator of Fe transport, UBC4/5 homolog, UBC5A, UBCH5, UBCH5A, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2 D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Met 147 |
| Accession | P51668 |
| Calculated Molecular Weight | 42.9 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | Ubiquitin-conjugating enzyme E2 D1（UBE2D1）belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This enzyme is closely related to a stimulator of iron transport (SFT); and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. |
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