Recombinant Human UBE2A Protein (GST & His Tag) (PKSH033322)
For research use only.
| Synonyms | HR6A, RAD6 Homolog A, RAD6A, UBE2A, Ubiquitin Carrier Protein A, Ubiquitin-Conjugating Enzyme E2 A, Ubiquitin-Protein Ligase A, hHR6A |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Cys152 |
| Accession | P49459 |
| Calculated Molecular Weight | 49.0 kDa |
| Observed Molecular Weight | 45 kDa |
| Tag | N-GST-His |
| Bio-activity | Not validated for activity |
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | Ubiquitin-Conjugating Enzyme E2 (UBE2A) is a member of the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is required for postreplication repair of UV-damaged DNA. UBE2A Interacts with RAD18 and WAC. |
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