Recombinant Human TUBB4A Protein (His Tag) (PKSH033158)

For research use only.
Synonyms | TUBB4, TUBB4A, TUBB5, Tubulin 5 Beta, Tubulin Beta-4 Chain, Tubulin Beta-4A Chain |
Species | Human |
Expression Host | E.coli |
Sequence | Met 1-Ala444 |
Accession | P04350 |
Calculated Molecular Weight | 51.7 kDa |
Observed Molecular Weight | 58 kDa |
Tag | N-His |
Bio-activity | Not validated for activity |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Histidine-HCl, 10% Trehalose, 100mM NaCl, 0.05% Tween 80, pH6.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background | Tubulin Beta-4A Chain (TUBB4A) is a cytoplasmic peptide containing 444 amino acids. TUBB4A is a member of the Tubulin family. Tubulin is the major constituent of microtubules. Tubulin is a dimer composed of one alpha and one beta tubulin molecule; there are many forms of beta tubulins, Beta II and Beta IV Tubulin are ubiquitously expressed. Beta-III Tubulin, also known as Tubulin Beta-4, is regarded as a neuron-specific marker. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. |
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