Recombinant Human TGF alpha protein(His Tag) (PKSH034146)
For research use only.
| Synonyms | ETGF, Sarcoma growth factor, TGF-type I |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Val 40-Ala 89 |
| Accession | P01135 |
| Calculated Molecular Weight | 6.5 kDa |
| Observed Molecular Weight | 7 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce 3T3 cells proliferation.The ED50 for this effect is <0.2 ng/mL.The specific activity of recombinant human TGF alpha is > 5 x 106 IU/mg. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. |
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